- Authors: Stephan, T., Roesch, A., Riedel, D., Jakobs, S.
- Title: Live-cell STED microscopy of mitochondrial cristae
- Journal: BioRxiv
- DOI: 10.1101/640920
Mitochondria are highly dynamic organelles that exhibit a complex inner architecture. They exhibit a smooth outer membrane and a highly convoluted inner membrane that forms invaginations called cristae. Imaging cristae in living cells poses a formidable challenge for light microscopy. Relying on a cell line stably expressing the mitochondrial protein Cox8A fused to the SNAP-tag and using STED (stimulated emission depletion) super-resolution microscopy, we demonstrate the visualization of cristae dynamics in cultivated human cells.