(27.06.2016) New article in BIOspektrum: "Reversibel schaltbare fluoreszierende Proteine für die Superauflösung"
Abstract: Super-resolution microscopy, or nanoscopy, enables the visualization of cellular structures inaccessible to conventional light microscopy. RESOLFT nanoscopy is especially suitable for the imaging of living cells. It requires fluorescent proteins (RSFPs) that can be repeatedly switched between a fluorescent and a non-fluorescent state by light. We have analyzed the molecular switching mechanisms and generated a family of RSFPs specifically tailored for live cell RESOLFT nanoscopy.
The cover shows RESOLFT (reversible saturable optical linear fluorescence transitions) microscopy on living cultured human U2OS cells. By gene modification with CRISPR/Cas9 the cells are able to express fusionproteins with the switchable fluorescent protein rsEGFP2 and Vimentin (in green, cell nuclei in blue). The magnification of the Vimentin filaments on the right shows the comparison between confocal (top) and RESOLFT microscopy (bottom)
The full article can be found in the 4/2016 issue of BIOspektrum or over the link in the title. (full article in german only)