Cookies Disclaimer

OK Our site saves small pieces of text information (cookies) on your device in order to deliver better content and for statistical purposes. You can disable the usage of cookies by changing the settings of your browser. By browsing our website without changing the browser settings you grant us permission to store that information on your device.

RSFPs

Reversibly switchable fluorescent proteins (RSFPs) are unusual and exciting proteins that may be reversibly switched by irradiation with light between a fluorescent (On) and a non-fluorescent (Off) state. They are enormously variable and therefore powerful probes for various forms of super-resolution microscopy including RESOLFT-microscopy. Using molecular design, we are changing and expanding the characteristics of RSFPs and investigating their properties on a molecular scale with a wide array of approaches including crystallography, Nuclear Magnetic Resonance (NMR), multi-dimension (MD) simulations and various forms of spectroscopy. We have created a palette of reversibly switching fluorescent proteins that cover a wide range of different colors, switching speeds and switching modes. These probes are used to study protein localization and dynamics on the nanoscale in living cells.

This endeavor is a joint effort with the Department of NanoBiophotonics at the Max Planck Institute for biophysical Chemistry in G├Âttingen.

References

Schnorrenberg S., Grotjohann T., Vorbr├╝ggen G., Herzig A., Hell S., Jakobs S.: "In vivo super-resolution RESOLFT microscopy of Drosophila melanogaster"
eLIFE e15567DOI 10.7554/eLife.15567Details

Grotjohann, T.,Testa, I.,Leutenegger, M.,Bock, H.,Urban, N.T.,Lavoie-Cardinal, F.,Willig, K.I.,Eggeling, C.,Jakobs, S. and Hell, S.W.: "Diffraction-unlimited all-optical imaging and writing with a photochromic GFP"
Nature 478 (7368), 204-208. DOI 10.1038/nature10497Details

Brakemann, T.,Stiel, A.C.,Weber, G.,Andresen, M.,Testa, I.,Grotjohann, T.,Leutenegger, M.,Plessmann, U.,Urlaub, H.,Eggeling, C.,Wahl, M.C.,Hell, S.W. and Jakobs, S.: "A reversibly photoswitchable GFP-like protein with fluorescence excitation decoupled from switching"
Nature Biotech. 29 (10), 942-U132. DOI 10.1038/nbt.1952Details

Andresen, M.,Stiel, A.C.,Foelling, J.,Wenzel, D.,Schoenle, A.,Egner, A.,Eggeling, C.,Hell, S.W. and Jakobs, S.: "Photoswitchable fluorescent proteins enable monochromatic multilabel imaging and dual color fluorescence nanoscopy"
Nature Biotech. 26 (9), 1035-1040. DOI 10.1038/nbt.1493Details