- Authors: Hell S. W., Willig K., Dyba M., Jakobs S., Kastrup L., & Westphal V.
- Title: Nanoscale resolution with focused light: STED and other RESOLFT microscopy concepts.
- Journal: Handbook Of Biological Confocal Microscopy 2006
- Volume: 571 - 579
- DOI: 10.1007/978-0-387-45524-2_31
Although most specifically labeled cellular constituents can readily be detected in a conventional fluorescence light microscope, their submicron-scale structure cannot be perceived. For example, despite the fact that many proteins of the inner mitochondrial membrane can be labeled by tagging with the green fluorescent protein (GFP), the cristae are too small to be represented in an image recorded with light.