Bibliographic Data
- Authors: Willig K.I., Kellner R.R., Medda R., Jakobs S., Hell S.W.
- Title: Nanoscale resolution in GFP-based microscopy
- Journal: Nature Methods
- Volume: 3
- Issue: 9
- Volume: 721-723
- DOI: 10.1038/nmeth922
Abstract
We report attainment of subdiffraction resolution using stimulated emission depletion (STED) microscopy with GFP-labeled samples. The similar to 70 nm lateral resolution attained in this study is demonstrated by imaging GFP-labeled viruses and the endoplasmic reticulum (ER) of a mammalian cell. Our results mark the advent of nanoscale biological microscopy with genetically encoded markers.