- Authors: Andresen M., Stiel A.C., Trowitzsch S., Weber G., Eggeling C., Wahl M.C., Hell S.W., Jakobs, S.
- Title: Structural basis for reversible photoswitching in Dronpa
- Journal: Proc. Natl. Acad. Sci. USA
- Volume: 104
- Issue: 32
- Volume: 13005-13009
- DOI: 10.1073/pnas.0700629104
Dronpa is a novel GFP-like fluorescent protein with exceptional light-controlled switching properties. It may be reversibly switched between a fluorescent on-state and a nonfluorescent off-state by irradiation with light. To elucidate the molecular basis of the switching mechanism, we generated reversibly switchable Dronpa protein crystals. Using these crystals we determined the elusive dark-state structure of Dronpa at 1.95-angstrom resolution. We found that the photoswitching results in a cis-trans isomerization of the chromophore accompanied by complex structural rearrangements of four nearby amino acid residues. Because of this cascade of intramolecular events, the chromophore is exposed to distinct electrostatic surface potentials, which are likely to influence the protonation equilibria at the chromophore. We suggest a comprehensive model for the light-induced switching mechanism, connecting a cascade of structural rearrangements with different protonation states of the chromophore.