Cookies Disclaimer

OK Our site saves small pieces of text information (cookies) on your device in order to deliver better content and for statistical purposes. You can disable the usage of cookies by changing the settings of your browser. By browsing our website without changing the browser settings you grant us permission to store that information on your device.


Bibliographic Data

  • Authors: Egner A., Geisler C., von Middendorff C., Bock H., Wenzel D., Medda R., Andresen M., Stiel A.C., Jakobs S., Eggeling C., Schönle A., Hell S.W.
  • Title: Fluorescence nanoscopy in whole cells by asynchronous localization of photoswitching emitters
  • Journal: Biophysical Journal
  • Volume: 93
  • Issue: 9
  • Volume: 3285-3290
  • DOI: 10.1529/biophysj.107.112201


We demonstrate nanoscale resolution in far-field fluorescence microscopy using reversible photoswitching and localization of individual fluorophores at comparatively fast recording speeds and from the interior of intact cells. These advancements have become possible by asynchronously recording the photon bursts of individual molecular switching cycles. We present images from the microtubular network of an intact mammalian cell with a resolution of 40 nm.