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Bibliographic Data

  • Authors: Foelling J., Bossi M., Bock H., Medda R., Wurm C.A., Hein B., Jakobs S., Eggeling C., Hell S.W.
  • Title: Fluorescence nanoscopy by ground-state depletion and single-molecule return
  • Journal: Nature Methods
  • Volume: 5
  • Volume: 943-945
  • DOI: 10.1038/nmeth.1257


We introduce far-field fluorescence nanoscopy with ordinary fluorophores based on switching the majority of them to a metastable dark state, such as the triplet, and calculating the position of those left or those that spontaneously returned to the ground state. Continuous widefield illumination by a single laser and a continuously operating camera yielded dual-color images of rhodamine-and fluorescent protein-labeled (living) samples, proving a simple yet powerful super-resolution approach.