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Publication

Bibliographic Data

  • Authors: Foelling, J.,Bossi, M.,Bock, H.,Medda, R.,Wurm, C.A.,Hein, B.,Jakobs, S.,Eggeling, C. and Hell, S.W.
  • Title: Fluorescence nanoscopy by ground-state depletion and single-molecule return
  • Journal: Nature Meth.
  • Volume: 5
  • Issue: 11
  • Volume: 943-945
  • DOI: 10.1038/nmeth.1257

Abstract

We introduce far-field fluorescence nanoscopy with ordinary fluorophores based on switching the majority of them to a metastable dark state, such as the triplet, and calculating the position of those left or those that spontaneously returned to the ground state. Continuous widefield illumination by a single laser and a continuously operating camera yielded dual-color images of rhodamine-and fluorescent protein-labeled (living) samples, proving a simple yet powerful super-resolution approach.