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Bibliographic Data

  • Authors: Bierwagen J., Testa I., Foelling J., Wenzel D., Jakobs S., Eggeling C., Hell S.W.
  • Title: Far-Field Autofluorescence Nanoscopy
  • Journal: Nano Letters
  • Volume: 10
  • Volume: 4249-4252
  • DOI: 10.1021/nl1027638


We demonstrate far-field optical imaging at the nanoscale with unlabeled samples Subdiffraaion resolution images of autofluorescent samples are obtained by depleting the ground state of natural fluorophores by transferring them to a metastable dark state and simultaneously localizing those fluorophores that are transiently returning Our approach is based on the insight that nanoscopy methods relying on stochastic single-molecule switching require only a single fluorescence on off cycle to yield an image, a condition fulfilled by various biomolecules The method is exemplified by recording label-free nanoscopy images of thylakoid membranes of spinach chloroplasts