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Publication

Bibliographic Data

  • Authors: Bierwagen, J.,Testa, I.,Foelling, J.,Wenzel, D.,Jakobs, S.,Eggeling, C. and Hell, S.W.
  • Title: Far-Field Autofluorescence Nanoscopy
  • Journal: Nano Lett.
  • Volume: 10
  • Issue: 10
  • Volume: 4249-4252
  • DOI: 10.1021/nl1027638

Abstract

We demonstrate far-field optical imaging at the nanoscale with unlabeled samples Subdiffraaion resolution images of autofluorescent samples are obtained by depleting the ground state of natural fluorophores by transferring them to a metastable dark state and simultaneously localizing those fluorophores that are transiently returning Our approach is based on the insight that nanoscopy methods relying on stochastic single-molecule switching require only a single fluorescence on off cycle to yield an image, a condition fulfilled by various biomolecules The method is exemplified by recording label-free nanoscopy images of thylakoid membranes of spinach chloroplasts