- Authors: Willig K.I., Stiel A.C., Brakemann T., Jakobs S., Hell S.W.
- Title: Dual-Label STED Nanoscopy of Living Cells Using Photochromism
- Journal: Nano Letters
- Volume: 11
- Volume: 3970-3973
- DOI: 10.1021/nl202290w
We demonstrate live-cell STED microscopy of two protein species using photochromic green fluorescent proteins as markers. The reversible photoswitching of two markers is implemented so that they can be discerned with a single excitation and STED wavelength and a single detection channel. Dual-label STED microscopy is shown in living mammalian cells.