Cookies Disclaimer

OK Our site saves small pieces of text information (cookies) on your device in order to deliver better content and for statistical purposes. You can disable the usage of cookies by changing the settings of your browser. By browsing our website without changing the browser settings you grant us permission to store that information on your device.

Publication

  • You are here:
  • Home
  • Publications
  • Willig K.I., Stiel A.C., Brakemann T., Jakobs S., Hell S.W.: Dual-Label STED Nanoscopy of Living ...

Bibliographic Data

  • Authors: Willig K.I., Stiel A.C., Brakemann T., Jakobs S., Hell S.W.
  • Title: Dual-Label STED Nanoscopy of Living Cells Using Photochromism
  • Journal: Nano Letters
  • Volume: 11
  • Volume: 3970-3973
  • DOI: 10.1021/nl202290w

Abstract

We demonstrate live-cell STED microscopy of two protein species using photochromic green fluorescent proteins as markers. The reversible photoswitching of two markers is implemented so that they can be discerned with a single excitation and STED wavelength and a single detection channel. Dual-label STED microscopy is shown in living mammalian cells.