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Bibliographic Data

  • Authors: Willig K.I., Stiel A.C., Brakemann T., Jakobs S., Hell S.W.
  • Title: Dual-Label STED Nanoscopy of Living Cells Using Photochromism
  • Journal: Nano Letters
  • Volume: 11
  • Volume: 3970-3973
  • DOI: 10.1021/nl202290w


We demonstrate live-cell STED microscopy of two protein species using photochromic green fluorescent proteins as markers. The reversible photoswitching of two markers is implemented so that they can be discerned with a single excitation and STED wavelength and a single detection channel. Dual-label STED microscopy is shown in living mammalian cells.