Cookies Disclaimer

OK Our site saves small pieces of text information (cookies) on your device in order to deliver better content and for statistical purposes. You can disable the usage of cookies by changing the settings of your browser. By browsing our website without changing the browser settings you grant us permission to store that information on your device.


Bibliographic Data

  • Authors: Kano, H.,Jakobs, S.,Nagorni, M. and Hell, S.W.
  • Title: Dual-color 4Pi-confocal microscopy with 3D-resolution in the 100 nm range
  • Journal: Ultramicroscopy
  • Volume: 90
  • Issue: 2-3
  • Volume: 207-213
  • DOI: 10.1016/s0304-3991(01)00132-2


We report the development of simultaneous two-color channel recording in 4Pi-confocal microscopy. A marked increase of spatial resolution over confocal microscopy becomes manifested in 4Pi-confocal three-dimensional (313) data stacks of dual-labeled objects. The fundamentally improved resolution is verified both with densely labeled fluorescence beads as well as with membrane labeled fixed Escherichia coli. The synergistic combination of dual-color 4Pi-confocal recording with image restoration results in dual-color imaging with a 3D resolution in the 100 nm range. (C) 2002 Elsevier Science B.V. All rights reserved.