- Authors: Chmyrov, A.,Keller, J.,Grotjohann, T.,Ratz, M.,d'Este, E.,Jakobs, S.,Eggeling, C. and Hell, S.W.
- Title: Nanoscopy with more than 100,000 'doughnuts'
- Journal: Nature Methods
- Volume: 10
- Issue: 8
- Volume: 737-740
- DOI: 10.1038/nmeth.2556
Highlighted in Nature Methods.
Journal Cover Nature Methods Aug. 2013.
We show that nanoscopy based on the principle called RESOLFT (reversible saturable optical fluorescence transitions) or nonlinear structured illumination can be effectively parallelized using two incoherently superimposed orthogonal standing light waves. The intensity minima of the resulting pattern act as 'doughnuts', providing isotropic resolution in the focal plane and making pattern rotation redundant. We super-resolved living cells in 120 mum x 100 mum-sized fields of view in <1 s using 116,000 such doughnuts.´