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Bibliographic Data

  • Authors: Jakobs S., Wurm C.A.
  • Title: Super-resolution microscopy of mitochondria
  • Journal: Current Opinion in Chemical Biology
  • Volume: 20
  • Volume: 9–15
  • DOI: 10.1016/j.cbpa.2014.03.019


Mitochondria, the powerhouses of the cell, are essential organelles in eukaryotic cells. With their complex inner architecture featuring a smooth outer and a highly convoluted inner membrane, they are challenging objects for microscopy. The diameter of mitochondria is generally close to the resolution limit of conventional light microscopy, rendering diffraction-unlimited super-resolution light microscopy (nanoscopy) for imaging submitochondrial protein distributions often mandatory. In this review, we discuss what can be expected when imaging mitochondria with conventional diffraction-limited and diffraction-unlimited microscopy. We provide an overview on recent studies using super-resolution microscopy to investigate mitochondria and discuss further developments and challenges in mitochondrial biology that might by addressed with these technologies in the future.