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Bibliographic Data

  • Authors: Dyba, M.,Jakobs, S. and Hell, S.W.
  • Title: Immunofluorescence stimulated emission depletion microscopy
  • Journal: Nature Biotech.
  • Volume: 21
  • Issue: 11
  • Volume: 1303-1304
  • DOI: 10.1038/nbt897
  • Journal Cover Nature Biotechnology Nov. 2003.


We report immunofluorescence imaging with a spatial resolution well beyond the diffraction limit. An axial resolution of 50 nm, corresponding to 1/16 of the irradiation wavelength of 793 nm, is achieved by stimulated emission depletion through opposing lenses. We have demonstrated not only that an antibody-tagged label is stable enough to be recorded in this microscopy mode, but also that subdiffraction resolution can be obtained using a standard immunofluorescence preparation.