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Publication

Bibliographic Data

  • Authors: Butkevich, A.N., Ta, H., Ratz, M., Stoldt, S., Jakobs, S., Belov, V.N., Hell, S.W.
  • Title: Two-Color 810 nm STED Nanoscopy of Living Cells with Endogenous SNAP-Tagged Fusion Proteins.
  • Journal: ACS Chemical Biology
  • Volume: 13
  • Issue: 2
  • Volume: 475 - 480
  • DOI: 10.1021/acschembio.7b00616

Abstract

A 810 nm STED nanoscopy setup and an appropriate combination of two fluorescent dyes (Si-rhodamine 680SiR and carbopyronine 610CP) have been developed for near-IR live-cell super-resolution imaging. Vimentin endogenously tagged using the CRISPR/Cas9 approach with the SNAP tag, together with a noncovalent tubulin label, provided reliable and cell-to-cell reproducible dual-color confocal and STED imaging of the cytoskeleton in living cells.