- Authors: Butkevich, A.N., Ta, H., Ratz, M., Stoldt, S., Jakobs, S., Belov, V.N., Hell, S.W.
- Title: Two-Color 810 nm STED Nanoscopy of Living Cells with Endogenous SNAP-Tagged Fusion Proteins.
- Journal: ACS Chemical Biology
- Volume: 13
- Issue: 2
- Volume: 475 - 480
- DOI: 10.1021/acschembio.7b00616
A 810 nm STED nanoscopy setup and an appropriate combination of two fluorescent dyes (Si-rhodamine 680SiR and carbopyronine 610CP) have been developed for near-IR live-cell super-resolution imaging. Vimentin endogenously tagged using the CRISPR/Cas9 approach with the SNAP tag, together with a noncovalent tubulin label, provided reliable and cell-to-cell reproducible dual-color confocal and STED imaging of the cytoskeleton in living cells.