Cookies Disclaimer

OK Our site saves small pieces of text information (cookies) on your device in order to deliver better content and for statistical purposes. You can disable the usage of cookies by changing the settings of your browser. By browsing our website without changing the browser settings you grant us permission to store that information on your device.


Bibliographic Data

  • Authors: Kamper M., Ta H., Jensen N.A., Hell S.W., Jakobs S.
  • Title: Near-infrared STED nanoscopy with an engineered bacterial phytochrome
  • Journal: Nature Communications
  • Volume: 9
  • Volume: 4762
  • DOI: 10.1038/s41467-018-07246-2
  • Access the Publication via Access the Publication via SN SharedIt


The near infrared (NIR) optical window between the cutoff for hemoglobin absorption at 650 nm and the onset of increased water absorption at 900 nm is an attractive, yet largely unexplored, spectral regime for diffraction-unlimited super-resolution fluorescence microscopy (nanoscopy). We developed the NIR fluorescent protein SNIFP, a bright and photostable bacteriophytochrome, and demonstrate its use as a fusion tag in live-cell microscopy and STED nanoscopy. We further demonstrate dual color red-confocal/NIR-STED imaging by co-expressing SNIFP with a conventional red fluorescent protein.