If you are interested in one of our plasmids, please contact Stefan Jakobs.
The following fluorescent proteins are available:
rsEGFP2
Grotjohann T., Testa I., Reuss M., Brakemann T., Eggeling C., Hell S.W., Jakobs S. (2012): "rsEGFP2 enables fast RESOLFT nanoscopy of living cells". ELife 1, e00248. DOI 10.7554/eLife.00248. Highlighted in The Scientist.
Dreiklang
Brakemann T., Stiel A.C., Weber G., Andresen M., Testa I., Grotjohann T., Leutenegger M., Plessmann U., Urlaub H., Eggeling C., Wahl M.C., Hell S.W.*, Jakobs S.* (2011): "A reversibly photoswitchable GFP-like protein with fluorescence excitation decoupled from switching". Nature Biotechnology 29, 942-947. DOI 10.1038/nbt.1952. (*shared corresponding authors)
Highlighted in Nature Biotechnoloy.
Journal Cover Nature Biotechnology Oct. 2011.
bsDronpa
Andresen M., Stiel A.C., Foelling J., Wenzel D., Schoenle A., Egner A., Eggeling C., Hell S.W., Jakobs, S. (2008): "Photoswitchable fluorescent proteins enable monochromatic multilabel imaging and dual color fluorescence nanoscopy". Nature Biotechnology 26, 1035-1040. DOI 10.1038/nbt.1493. Highlighted in Nature Methods and Nature Chemical Biology.
Padron
Andresen M., Stiel A.C., Foelling J., Wenzel D., Schoenle A., Egner A., Eggeling C., Hell S.W., Jakobs, S. (2008): "Photoswitchable fluorescent proteins enable monochromatic multilabel imaging and dual color fluorescence nanoscopy". Nature Biotechnology 26, 1035-1040. DOI 10.1038/nbt.1493. Highlighted in Nature Methods and Nature Chemical Biology.
Padron GE
Willig K.I., Stiel A.C., Brakemann T., Jakobs S., Hell S.W. (2011): "Dual-Label STED Nanoscopy of Living Cells Using Photochromism". Nano Letters 11, 3970-3973. DOI 10.1021/nl202290w
rsCherryRev 1.4
Lavoie-Cardinal F., Jensen N.A., Westphal V., Stiel A.C., Chmyrov A., Bierwagen J., Testa I., Jakobs S., Hell S.W. (2014): "Two-Color RESOLFT Nanoscopy with Green and Red Fluorescent Photochromic Proteins". ChemPhysChem 15, 655-663. DOI 10.1002/cphc.201301016. Journal Cover ChemPhysChem Mar. 2014.